https://www.perrimangroup.co.uk/about-us daily 0.75 2015-09-15 https://www.perrimangroup.co.uk/research daily 1.0 2020-02-27 https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e57b2274e2df00530a6d1da/1582805556625/nature+cover Home https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e540933e89a2d3c693d9c8a/1582565742408/FigHDR3_CS.png Home https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5d94a076e1ddf776827d9dd7/1582479861319/Functional%2Bscaffold.jpg Home Confocal fluorescence microscope image of a tissue scaffold fragment after covalent attachment of enhanced green fluorescent protein molecules. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e583c51bb56806311256b6b/1582841017078/Col+IV+co+cult+40x.png Home https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e57ae78fbd70e218a5f16d5/1582804617280/Blueprint_portrait.png Home 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https://www.perrimangroup.co.uk/publications/insight-into-the-structure-and-activity-of-surfaceengineered-lipase-biofluids monthly 0.5 2019-08-30 https://www.perrimangroup.co.uk/publications/a-composite-hydrogel-scaffold-permits-selforganization-and-matrix-deposition-by-cocultured-human-glomerular-cells monthly 0.5 2019-08-30 https://www.perrimangroup.co.uk/publications/3d-bioprinting-the-emergence-of-programmable-biodesign monthly 0.5 2019-08-30 https://www.perrimangroup.co.uk/groupmembers daily 0.75 2021-02-05 https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e52e2196dd0756fb8009934/1582490157766/labwork7.jpg All Members https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4fdee0e28b674d653fd273/1582982633434/ All Members - Professor Adam Perriman https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4d83d35864f4372b4903e7/1582983018422/ All Members - Dr Ben Carter I worked in the Perriman Group from September 2014 – March 2018. My PhD, entitled 'Constructs for the detoxification of organophosphorous compounds', involved developing the first artificial membrane-binding enzymes. Using OpdA, an OP-degrading enzyme that evolved in soil bacteria, I was able to generate enzymatically-active constructs that spontaneously bound to cell membranes. I now work for CytoSeek, a spin-out from the Perriman group that is commercialising artificial membrane-binding protein technology to address unmet needs in cell-therapy treatments for cancers. For more information, visit cytoseek.uk. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4fdb65db6bdf4d944f8835/1582296503520/DavidC.png All Members - Dr David Coe https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4d80a33f73540486ffaaac/1582548657651/ All Members - Dr Sara Correia Carreira I'm developing the next generation of laboratory models of human skin with the aim of reducing animal testing. A major limitation of current skin models is that they don’t mimic the stretching and bending that skin experiences in real life. It is important to incorporate these motions because they can influence how substances penetrate through skin, or how wearable devices perform when skin is moving. In my research, I'm interfacing tissue engineered skin with soft robotic actuators, which function like artificial muscles that can stretch and bend the skin. Delivering mechanical stimulation to lab-grown skin can also improve the quality of skin grafts used to treat burns and wounds. Currently, these grafts are grown in the lab without ever experiencing the mechanical stresses they will encounter once they are applied to the patient. This means they don’t build up the toughness and structure of natural skin, which may be a reason for skin graft failures, such as tears. Therefore, I am also working towards engineering better skin grafts. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4bd3ce79fe5b341b7d3b96/1582986311415/RaquelCS.jpg.png All Members - Dr Raquel Cruz Samperio Macromolecular assembly of protein fusions to stem cells for cardiac therapy. Designing and optimising multi-module protein fusions to enhance the therapeutic performance of stem cells and their homing to infarcted tissue. These proteins contain one module that anchors to the cell membrane of stem cells by ionic interaction, whilst the other module is an acceptor for specific fusion to other proteins (e.g. fluorescent tags, transcription factors, etc.). https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4c4ca320c52d785aaf880d/1582296503631/ All Members - Dr Graham Day My project involves the biophysical and structural characterisation of phosphotriesterases, which hydrolyse neurotoxic organophosphorus compounds found in chemical warfare agents and pesticides. Moreover, these enzymes are re-engineered to facilitate complexation to polymer surfactants to generate hierarchically self-assembled materials such as films or composite textiles. These robust materials exhibit the properties of the constituent proteins and offer new solutions to protection from and decontamination of organophosphorus compounds. I am also exploring new enzyme–biopolymer conjugation strategies to increase the catalytic activity or substrate range of these enzymes. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4c41808861a57e42526419/1582296503627/AndreaDG.png All Members - Andrea Diaz Gaxiola I am working on the manufacturing of light-controlled soft robots. These are not the classically known robots made of steel and wires. On the contrary, they are hydrogels made soft and squishy, yet tough and elastic. The greatest feature is their application as smart scaffolds for tissue engineering. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4bd3b0065dc80ce71c07a9/1583186349606/CorriganH.png All Members - Corrigan Hicks My project involves the development of artificial membrane binding proteins (AMBPs) for the modification of synthetic vesicles to enhance in vivo cardiovascular repair. My project involves the rational design and comprehensive biophysical characterisation of both the vesicles, AMBPs and modified vesicles. This work is a step towards building a modular homing system to target synthetic vesicles towards various specific sites in vivo. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4bd3a971f6217a28f2949f/1612531278943/BethH.png All Members - Beth Hickton My project is a dstl industry-sponsored collaboration between the departments of Aerospace and Biomedical Engineering. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4c41ff2b169d25f3733085/1582480894463/person_teal.png All Members - Agata Jakimowicz My research project is focused on the development of a smart microporous biological 3D printable ink (bioink) that can be used in both in vitro and in vivo tissue engineering. The structural component of the bioink is a viscoelastic hydrogel that has a highly tuneable temperature-dependent phase transition. In practice, this allows it to be extruded from nozzles (e.g. a needle) as a liquid at room temperature, which subsequently transforms into a self-supporting hydrogel at body temperature. Housed within this biomaterial-based hydrogel, is a cell-free expression system, which can be developed to produce growth factors that are essential for bone healing. Significantly, the delivery of these factors will be controlled using transcription regulation, such that the growth factor expression levels can be systematically modulated. The resulting smart bioink will be 3D printed in the presence of mesenchymal stem cells to control differentiation. This method of stem cell differentiation stimulation into osteoblasts has potential as a powerful technique in tissue engineering research and as a clinical tool for bone fracture repair. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4bd3cd6d8433076e1b4ebc/1582296504120/PeterJ.png All Members - Peter Johnson 3D response of cancer to chemotherapies can be modelled using cancer spheroids, which grow from single cancer cells in a 3D matrix. High throughput bioprinting, coupled with high content microscopy and image analysis, allows for the screening of different therapeutic combinations against thousands of cancer spheroids in a controlled manner. In the future, this approach could be used to reduce the need for animals in drug testing, or to personalise cancer therapies for patients by analysing treatment combinations to find an optimal therapy. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4bd3bc47efbd6a69b7ed8d/1582984057384/GeorgeK.png All Members - George Klemperer My research work is in the area of 3D-printable engineered living materials (ELMs). ELMs are a type of biohybrid material that combine whole living cells with non-biological components/scaffolds. The idea is to combine the best of both worlds. The autonomous capabilities of cells with the tractability of synthetic materials (may they be polymers/gels/ceramics/metals etc.) to create materials with novel biologically-derived feedback properties and embedded intelligence. In my project, I aim develop a 3D printable E. coli-laden bioink ELM. This will become a new platform for patterned bacterial microreactor synthesis (constructs augmented with the abilities of transformed E. coli). Current bacterial microreactor applications being investigated include organophosphate bioremediation and hybrid gel synthesis. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4bd3e66ddc335ff290b021/1582296504233/WillM.jpg.png All Members - William Macalester Periodontitis is an oral disease characterised by the persistent inflammation of periodontal tissues which, if left untreated, ultimately leads to alveolar bone dissolution and tooth loss. My project aims to create an in vitro model of the human periodontium in order to elucidate potential growth mechanisms which may inform the development of regenerative therapies. This work combines bioprinting and tissue engineering approaches to generate a 3D tissue model amenable to high throughput testing. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4c41818b747830b7f58d5c/1582487412233/SaraM.png All Members - Dr Sara Menegatti Trained as a Stem Cell Biologist, I joined CytoSeek and its mission to improve cell therapies by using a unique cell membrane augmentation technology. My aim is to develop in vitro and in vivo assays to assess the efficacy of our therapeutic in the fields of oncology and regenerative medicine. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4bd3dacbc08f59eeb57e0b/1582548749043/TomR.png All Members - Dr Thomas Richardson My doctoral and postdoctoral work has centered around building a 3D model of the renal glomerular filtration barrier, using conditionally immortalised podocytes and endothelial cells first established at the University of Bristol. I broadly followed two approaches to achieve this aim, one using 3D bioprinting and the other using fibrin gels and protein engineering. In the latter, I co-developed a method generating 3D fibrin scaffolds from purified and modified thrombin. By chemically modifying thrombin so it had a surfactant corona, the enzyme could be embedded in the membrane of several cell types. In the presence of fibrinogen in solution, this membrane bound thrombin would form a hydrogel from the very surface of the cells, serving as a kind of extracellular matrix (ECM) mimic. Conditionally immortalised podocytes and endothelial cells in these 3D fibrin gels produced ECM specific to the glomerular basement membrane, which was verified using lightsheet microscopy. The second major aim of my PhD was to develop new 3D bioprinting methods to build a 3D renal model. I have also developed methodologies relating to fluorescence imaging (namely confocal microscopy), image processing, electron microscopy and immunohistochemistry. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/601d44e7e65d787cd60a869a/1612531497476/ValeriaS.jpg All Members - Valeria Sandoval Torres Cytoseek Partnered Project - Adoptive Cell Therapies for Cancer https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4bd3c701c72f4e3e82aad6/1582296504724/MarkS.png All Members - Mark Shannon I’m working on the fabrication of engineered living materials towards the development of bioreactors. This project involves the 3D bioprinting of bacteria laden hydrogels that can be precisely deposited into predesigned architectures. These structures both shelter the cells and maintain their metabolic function. The genetic tractability of bacteria permits the expression of a wide range of recombinant enzymes to tailor these materials for bespoke application in bioremediation. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4ec0020a541b68f725b764/1582548667843/JennaS.jpg All Members - Dr Jenna Shapiro https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4c531b9acc280f2920cf7b/1582487457364/Person+gray.png All Members - Thomas Taylor Cytoseek Partnered Project https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4c53271a75040c0b0d8114/1582487436350/Person_white.png All Members - Ximena Vasto Anzaldo Cytoseek Partnered Project. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4c352eab57597f6de42deb/1582052688316/2020WIS.png All Members https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4c4e061a75040c0b0c3873/1589196472475/Person_rose.png All Members - Ioannis Zampetakis The research project focuses on the development of novel composite materials using Cactus (Opuntia ficus-indica) fibres for impact energy dissipation and bone tissue engineering applications. This type of natural fibres has shown interesting deformation mechanics and energy dissipating potential under cyclic fatigue loading. We aim to characterize the natural fibres on a multiscale level to gain an understanding of their mechanical properties and use them as design guidelines for the development of novel polymeric composites and assessing their potential in energy dissipation and bone regeneration applications. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4fee041387b9765714f244/1582710414973/TomG.png All Members - Dr Thomas Green As a PhD student in the Perriman Lab I was primarily interested in augmenting the functionality of stem cells without modifying their genomes; particularly focusing on homing to damaged heart tissue following myocardial infarction. I accomplished this by constructing an artificial membrane binding molecule which incorporated a repurposed bacterial adhesion molecule. This novel construct bound to immobilized fibronectin with greater specificity and affinity than a comparable antibody, and demonstrated increased mesenchymal stem cell homing to cardiac tissue in a mouse model. This methodology paved the way for further experiments in the group using similar systems. After completing my PhD research I joined Professor Perriman’s spin-out company Cytoseek as a founding member and now work towards commercialising the technology developed by myself and others in the academic group https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e555ff921592f1f698f65f7/1582807394026/MedelineBurke.png All Members - Dr Madeline Burke My project which encompassed a wide range of interdisciplinary topics, including scaffold design for tissue engineering, the culture of disease models for kidney fibrosis, and 3D bioprinting of cells. Successfully graduated in 2017 and am now working as a Science Policy advisor in Westminster https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e556001c253f84cdc25b70c/1582653449329/KirsLeVay.png All Members https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e556121035f7f739e97dfd4/1582654742201/JamesArmstrong.png All Members - Dr James Armstrong https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e5564e7f4d63a75132e73d0/1582655216430/MonikaJakimowicz.png All Members - Dr Monika Jakimowicz https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e5566cc0c0e830ca4ac804f/1582655266993/RosaliaCua.png All Members - Dr Rosalia Cuahtecontzi https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e5568f27b18e31d4d100801/1582655783901/ All Members - Dr Robert Deller https://www.perrimangroup.co.uk/gallery daily 0.75 2020-02-27 https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e556d2c3d2f9f7b147b9ff4/1582656947068/labwork5.jpg Group Gallery https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e555dee1330ac6a2d9ea073/5e555df949a12573884fa0dc/1582653088222/ Group Gallery - Graduation Day Dr Ben Carter and Dr Rosalia Cuahtecontzi on Dr Graham Day’s graduation Ceremony https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e555dee1330ac6a2d9ea073/5e555e7521592f1f698f00e0/1582653294662/IMG_0986_edited.jpg Group Gallery - Spun Out Graduation Day for the Alumni who now run the Spin Out: Cytoseek. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e555dee1330ac6a2d9ea073/5e5562d8c253f84cdc266ccb/1582654175211/DrJonathanFurze.jpg Group Gallery https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e555dee1330ac6a2d9ea073/5e5562f07b18e31d4d0e8c5e/1582654201368/IMG_0434_edited.jpg Group Gallery https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e555dee1330ac6a2d9ea073/5e556a91beef496cb74831c8/1582656215645/2020WIS.png Group Gallery - Women in Science Day https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e555dee1330ac6a2d9ea073/5e581a8fa337185b13684668/1582832301845/IMG_2812_edited.jpg Group Gallery https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e555dee1330ac6a2d9ea073/5e581ac519f9d5146a390761/1582832335991/IMG_2803_edited.jpg Group Gallery https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e555dee1330ac6a2d9ea073/5e581addb31e10301c8c409c/1582832358157/PSX_20200225_190015.jpg Group Gallery https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e555dee1330ac6a2d9ea073/5e581af31106b353433f2363/1582832405220/IMG_2888_edited.jpg Group Gallery https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e555dee1330ac6a2d9ea073/5e581b2c110a412c3f74c0cf/1582832459213/IMG_2640_edited.jpg Group Gallery https://www.perrimangroup.co.uk/welcome daily 0.75 2020-02-25 https://www.perrimangroup.co.uk/adam-perriman daily 0.75 2020-05-11 https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e531456dbfcb2688e3cb940/1582503012564/Adam_BW3_round.jpg.png Professor Adam Perriman - Biography Adam Perriman is a Professor of Bioengineering at the School of Cellular and Molecular Medicine, University of Bristol, and Director of the Bristol Centre for Bioprinting. He is also the founder of the cell therapy technology company CytoSeek. Internationally distinguished for his pioneering research on the construction and study of novel synthetic biomolecular systems, and his research interests span the fields of synthetic biology, biophysics and tissue engineering. His research is frequently featured in top-tier journals, including Nature Chemistry, Nature Communications, the Journal of the American Chemical Society, Small, and Advanced Materials. His research into the development of novel biomaterials has also generated extensive media coverage and has been featured nationally in RSC’s Chemistry World, The Chemical Engineer, New Scientist, and internationally in Nature, Nature Chemistry and Chemical and Engineering News (C&EN). This research also led to nationally-broadcast interviews on BBC4. His significant advances in these areas have resulted in invited presentations at major international conferences, including the Materials Research Society (MRS) meetings and Gordon conferences. His contributions to the field of interdisciplinary science led to him being named a Wellcome Trust Frontiers Innovator in 2015, and in 2016, he was awarded the prestigious British Biophysical Society Young Investigator's Award and Medal. In 2019, he was named a UK Research and Innovation Future Leaders Fellow. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e531697576c4c1eab8ba02e/1582503591228/Green70_lab_banner.png Professor Adam Perriman https://www.perrimangroup.co.uk/alumni daily 0.75 2020-02-27 https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4bd3a971f6217a28f2949f/1612531278943/BethH.png Alumni - Beth Hickton My project is a dstl industry-sponsored collaboration between the departments of Aerospace and Biomedical Engineering. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4bd3b0065dc80ce71c07a9/1583186349606/CorriganH.png Alumni - Corrigan Hicks My project involves the development of artificial membrane binding proteins (AMBPs) for the modification of synthetic vesicles to enhance in vivo cardiovascular repair. My project involves the rational design and comprehensive biophysical characterisation of both the vesicles, AMBPs and modified vesicles. This work is a step towards building a modular homing system to target synthetic vesicles towards various specific sites in vivo. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4bd3bc47efbd6a69b7ed8d/1582984057384/GeorgeK.png Alumni - George Klemperer My research work is in the area of 3D-printable engineered living materials (ELMs). ELMs are a type of biohybrid material that combine whole living cells with non-biological components/scaffolds. The idea is to combine the best of both worlds. The autonomous capabilities of cells with the tractability of synthetic materials (may they be polymers/gels/ceramics/metals etc.) to create materials with novel biologically-derived feedback properties and embedded intelligence. In my project, I aim develop a 3D printable E. coli-laden bioink ELM. This will become a new platform for patterned bacterial microreactor synthesis (constructs augmented with the abilities of transformed E. coli). Current bacterial microreactor applications being investigated include organophosphate bioremediation and hybrid gel synthesis. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4bd3c701c72f4e3e82aad6/1582296504724/MarkS.png Alumni - Mark Shannon I’m working on the fabrication of engineered living materials towards the development of bioreactors. This project involves the 3D bioprinting of bacteria laden hydrogels that can be precisely deposited into predesigned architectures. These structures both shelter the cells and maintain their metabolic function. The genetic tractability of bacteria permits the expression of a wide range of recombinant enzymes to tailor these materials for bespoke application in bioremediation. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4bd3cd6d8433076e1b4ebc/1582296504120/PeterJ.png Alumni - Peter Johnson 3D response of cancer to chemotherapies can be modelled using cancer spheroids, which grow from single cancer cells in a 3D matrix. High throughput bioprinting, coupled with high content microscopy and image analysis, allows for the screening of different therapeutic combinations against thousands of cancer spheroids in a controlled manner. In the future, this approach could be used to reduce the need for animals in drug testing, or to personalise cancer therapies for patients by analysing treatment combinations to find an optimal therapy. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4bd3ce79fe5b341b7d3b96/1582986311415/RaquelCS.jpg.png Alumni - Dr Raquel Cruz Samperio Macromolecular assembly of protein fusions to stem cells for cardiac therapy. Designing and optimising multi-module protein fusions to enhance the therapeutic performance of stem cells and their homing to infarcted tissue. These proteins contain one module that anchors to the cell membrane of stem cells by ionic interaction, whilst the other module is an acceptor for specific fusion to other proteins (e.g. fluorescent tags, transcription factors, etc.). https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4bd3dacbc08f59eeb57e0b/1582548749043/TomR.png Alumni - Dr Thomas Richardson My doctoral and postdoctoral work has centered around building a 3D model of the renal glomerular filtration barrier, using conditionally immortalised podocytes and endothelial cells first established at the University of Bristol. I broadly followed two approaches to achieve this aim, one using 3D bioprinting and the other using fibrin gels and protein engineering. In the latter, I co-developed a method generating 3D fibrin scaffolds from purified and modified thrombin. By chemically modifying thrombin so it had a surfactant corona, the enzyme could be embedded in the membrane of several cell types. In the presence of fibrinogen in solution, this membrane bound thrombin would form a hydrogel from the very surface of the cells, serving as a kind of extracellular matrix (ECM) mimic. Conditionally immortalised podocytes and endothelial cells in these 3D fibrin gels produced ECM specific to the glomerular basement membrane, which was verified using lightsheet microscopy. The second major aim of my PhD was to develop new 3D bioprinting methods to build a 3D renal model. I have also developed methodologies relating to fluorescence imaging (namely confocal microscopy), image processing, electron microscopy and immunohistochemistry. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4bd3e66ddc335ff290b021/1582296504233/WillM.jpg.png Alumni - William Macalester Periodontitis is an oral disease characterised by the persistent inflammation of periodontal tissues which, if left untreated, ultimately leads to alveolar bone dissolution and tooth loss. My project aims to create an in vitro model of the human periodontium in order to elucidate potential growth mechanisms which may inform the development of regenerative therapies. This work combines bioprinting and tissue engineering approaches to generate a 3D tissue model amenable to high throughput testing. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4c352eab57597f6de42deb/1582052688316/2020WIS.png Alumni https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4c41818b747830b7f58d5c/1582487412233/SaraM.png Alumni - Dr Sara Menegatti Trained as a Stem Cell Biologist, I joined CytoSeek and its mission to improve cell therapies by using a unique cell membrane augmentation technology. My aim is to develop in vitro and in vivo assays to assess the efficacy of our therapeutic in the fields of oncology and regenerative medicine. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4c41808861a57e42526419/1582296503627/AndreaDG.png Alumni - Andrea Diaz Gaxiola I am working on the manufacturing of light-controlled soft robots. These are not the classically known robots made of steel and wires. On the contrary, they are hydrogels made soft and squishy, yet tough and elastic. The greatest feature is their application as smart scaffolds for tissue engineering. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4c41ff2b169d25f3733085/1582480894463/person_teal.png Alumni - Agata Jakimowicz My research project is focused on the development of a smart microporous biological 3D printable ink (bioink) that can be used in both in vitro and in vivo tissue engineering. The structural component of the bioink is a viscoelastic hydrogel that has a highly tuneable temperature-dependent phase transition. In practice, this allows it to be extruded from nozzles (e.g. a needle) as a liquid at room temperature, which subsequently transforms into a self-supporting hydrogel at body temperature. Housed within this biomaterial-based hydrogel, is a cell-free expression system, which can be developed to produce growth factors that are essential for bone healing. Significantly, the delivery of these factors will be controlled using transcription regulation, such that the growth factor expression levels can be systematically modulated. The resulting smart bioink will be 3D printed in the presence of mesenchymal stem cells to control differentiation. This method of stem cell differentiation stimulation into osteoblasts has potential as a powerful technique in tissue engineering research and as a clinical tool for bone fracture repair. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4c4ca320c52d785aaf880d/1582296503631/ Alumni - Dr Graham Day My project involves the biophysical and structural characterisation of phosphotriesterases, which hydrolyse neurotoxic organophosphorus compounds found in chemical warfare agents and pesticides. Moreover, these enzymes are re-engineered to facilitate complexation to polymer surfactants to generate hierarchically self-assembled materials such as films or composite textiles. These robust materials exhibit the properties of the constituent proteins and offer new solutions to protection from and decontamination of organophosphorus compounds. I am also exploring new enzyme–biopolymer conjugation strategies to increase the catalytic activity or substrate range of these enzymes. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4c4e061a75040c0b0c3873/1589196472475/Person_rose.png Alumni - Ioannis Zampetakis The research project focuses on the development of novel composite materials using Cactus (Opuntia ficus-indica) fibres for impact energy dissipation and bone tissue engineering applications. This type of natural fibres has shown interesting deformation mechanics and energy dissipating potential under cyclic fatigue loading. We aim to characterize the natural fibres on a multiscale level to gain an understanding of their mechanical properties and use them as design guidelines for the development of novel polymeric composites and assessing their potential in energy dissipation and bone regeneration applications. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4c531b9acc280f2920cf7b/1582487457364/Person+gray.png Alumni - Thomas Taylor Cytoseek Partnered Project https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4c53271a75040c0b0d8114/1582487436350/Person_white.png Alumni - Ximena Vasto Anzaldo Cytoseek Partnered Project. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4d80a33f73540486ffaaac/1582548657651/ Alumni - Dr Sara Correia Carreira I'm developing the next generation of laboratory models of human skin with the aim of reducing animal testing. A major limitation of current skin models is that they don’t mimic the stretching and bending that skin experiences in real life. It is important to incorporate these motions because they can influence how substances penetrate through skin, or how wearable devices perform when skin is moving. In my research, I'm interfacing tissue engineered skin with soft robotic actuators, which function like artificial muscles that can stretch and bend the skin. Delivering mechanical stimulation to lab-grown skin can also improve the quality of skin grafts used to treat burns and wounds. Currently, these grafts are grown in the lab without ever experiencing the mechanical stresses they will encounter once they are applied to the patient. This means they don’t build up the toughness and structure of natural skin, which may be a reason for skin graft failures, such as tears. Therefore, I am also working towards engineering better skin grafts. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4d83d35864f4372b4903e7/1582983018422/ Alumni - Dr Ben Carter I worked in the Perriman Group from September 2014 – March 2018. My PhD, entitled 'Constructs for the detoxification of organophosphorous compounds', involved developing the first artificial membrane-binding enzymes. Using OpdA, an OP-degrading enzyme that evolved in soil bacteria, I was able to generate enzymatically-active constructs that spontaneously bound to cell membranes. I now work for CytoSeek, a spin-out from the Perriman group that is commercialising artificial membrane-binding protein technology to address unmet needs in cell-therapy treatments for cancers. For more information, visit cytoseek.uk. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4ec0020a541b68f725b764/1582548667843/JennaS.jpg Alumni - Dr Jenna Shapiro https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4fdb65db6bdf4d944f8835/1582296503520/DavidC.png Alumni - Dr David Coe https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4fdee0e28b674d653fd273/1582982633434/ Alumni - Professor Adam Perriman https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4fee041387b9765714f244/1582710414973/TomG.png Alumni - Dr Thomas Green As a PhD student in the Perriman Lab I was primarily interested in augmenting the functionality of stem cells without modifying their genomes; particularly focusing on homing to damaged heart tissue following myocardial infarction. I accomplished this by constructing an artificial membrane binding molecule which incorporated a repurposed bacterial adhesion molecule. This novel construct bound to immobilized fibronectin with greater specificity and affinity than a comparable antibody, and demonstrated increased mesenchymal stem cell homing to cardiac tissue in a mouse model. This methodology paved the way for further experiments in the group using similar systems. After completing my PhD research I joined Professor Perriman’s spin-out company Cytoseek as a founding member and now work towards commercialising the technology developed by myself and others in the academic group https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e555ff921592f1f698f65f7/1582807394026/MedelineBurke.png Alumni - Dr Madeline Burke My project which encompassed a wide range of interdisciplinary topics, including scaffold design for tissue engineering, the culture of disease models for kidney fibrosis, and 3D bioprinting of cells. Successfully graduated in 2017 and am now working as a Science Policy advisor in Westminster https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e556001c253f84cdc25b70c/1582653449329/KirsLeVay.png Alumni https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e556121035f7f739e97dfd4/1582654742201/JamesArmstrong.png Alumni - Dr James Armstrong https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e5564e7f4d63a75132e73d0/1582655216430/MonikaJakimowicz.png Alumni - Dr Monika Jakimowicz https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e5566cc0c0e830ca4ac804f/1582655266993/RosaliaCua.png Alumni - Dr Rosalia Cuahtecontzi https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e5568f27b18e31d4d100801/1582655783901/ Alumni - Dr Robert Deller https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/601d44e7e65d787cd60a869a/1612531497476/ValeriaS.jpg Alumni - Valeria Sandoval Torres Cytoseek Partnered Project - Adoptive Cell Therapies for Cancer https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e555965c6dc64660bb84ef5/1582651755993/banner_edited.jpg Alumni https://www.perrimangroup.co.uk/collaborations daily 0.75 2020-02-29 https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e55a07f59a6300d9dbd5828/1582669959576/SynBioCDT_white.png Partners & Sponsors https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e543e0b4f74387f5e292a63/1582579214547/Cytoseeklogo.png Partners & Sponsors https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e559ffd6c2fb67a0ee74274/1582669832195/bsb_strapline_white.png Partners & Sponsors https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e55a38f31985e5b5069e5e8/1582670743318/Unit-DX-Logo-White.png Partners & Sponsors https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e559da696f3913deb5d6a10/1582669223448/bbsrc.png Partners & Sponsors https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e559cf83d9b960eaedbc679/1582673406176/UKRI_EPSR_Council-Logo_Horiz-Grayscale%5BW%5D.png Partners & Sponsors https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e559c9b012a8f6d3cfbbe95/1582673588579/GSK_Logo-Symbol_WHITE.png Partners & Sponsors https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e55a15a31985e5b506953d5/1582670178736/BCFN+COLOUR_white.png Partners & Sponsors https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e5a66e7483fcf20332391d4/1582982911174/Cellink_logo-1.png Partners & Sponsors https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e557654c9510143c968e5f2/1582659159633/ Partners & Sponsors https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e55a31659a6300d9dbe0708/1582670669520/lab_4_green.png Partners & Sponsors https://www.perrimangroup.co.uk/contact daily 0.75 2020-02-25 https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e55ad0b52ad19666931819d/1582673316339/Blueprint2.png Contact https://www.perrimangroup.co.uk/bioprinting daily 0.75 2020-02-27 https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e583a1f750bff750e6df3e4/1582840393922/O+-+Col+1+%2B+phalloidin+%2B+DAPI.lif+-+Image008.png Bioprinting https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e53c403976c87268c3689e8/1582548025979/Tweezed+square_2.png Bioprinting https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e53c94c8ff9f5167fcf02e6/1582549356026/Armstrong_et_al-2016-Advanced_Healthcare_Materials-1.jpg Bioprinting https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e58380907744a52ac6628ab/1582839823357/spheroids_flasecolor.png Bioprinting https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e583b258fa565211041a004/1582840623270/E+-+Col+1+%2B+phalloidin+DAPI.lif+-+Image004-Processed.png Bioprinting Undifferentiated Control https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e5839010fd9291402b58ed4/1582840075558/different+line+spheroids.png Bioprinting Spheroids from different cell lines. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e53cceeaf3a3e1585dfd9d1/1582550266474/banner_bioprint_2.png Bioprinting https://www.perrimangroup.co.uk/students daily 0.75 2020-02-20 https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4bd3a971f6217a28f2949f/1612531278943/BethH.png Students - Beth Hickton My project is a dstl industry-sponsored collaboration between the departments of Aerospace and Biomedical Engineering. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4bd3b0065dc80ce71c07a9/1583186349606/CorriganH.png Students - Corrigan Hicks My project involves the development of artificial membrane binding proteins (AMBPs) for the modification of synthetic vesicles to enhance in vivo cardiovascular repair. My project involves the rational design and comprehensive biophysical characterisation of both the vesicles, AMBPs and modified vesicles. This work is a step towards building a modular homing system to target synthetic vesicles towards various specific sites in vivo. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4bd3bc47efbd6a69b7ed8d/1582984057384/GeorgeK.png Students - George Klemperer My research work is in the area of 3D-printable engineered living materials (ELMs). ELMs are a type of biohybrid material that combine whole living cells with non-biological components/scaffolds. The idea is to combine the best of both worlds. The autonomous capabilities of cells with the tractability of synthetic materials (may they be polymers/gels/ceramics/metals etc.) to create materials with novel biologically-derived feedback properties and embedded intelligence. In my project, I aim develop a 3D printable E. coli-laden bioink ELM. This will become a new platform for patterned bacterial microreactor synthesis (constructs augmented with the abilities of transformed E. coli). Current bacterial microreactor applications being investigated include organophosphate bioremediation and hybrid gel synthesis. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4bd3c701c72f4e3e82aad6/1582296504724/MarkS.png Students - Mark Shannon I’m working on the fabrication of engineered living materials towards the development of bioreactors. This project involves the 3D bioprinting of bacteria laden hydrogels that can be precisely deposited into predesigned architectures. These structures both shelter the cells and maintain their metabolic function. The genetic tractability of bacteria permits the expression of a wide range of recombinant enzymes to tailor these materials for bespoke application in bioremediation. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4bd3cd6d8433076e1b4ebc/1582296504120/PeterJ.png Students - Peter Johnson 3D response of cancer to chemotherapies can be modelled using cancer spheroids, which grow from single cancer cells in a 3D matrix. High throughput bioprinting, coupled with high content microscopy and image analysis, allows for the screening of different therapeutic combinations against thousands of cancer spheroids in a controlled manner. In the future, this approach could be used to reduce the need for animals in drug testing, or to personalise cancer therapies for patients by analysing treatment combinations to find an optimal therapy. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4bd3ce79fe5b341b7d3b96/1582986311415/RaquelCS.jpg.png Students - Dr Raquel Cruz Samperio Macromolecular assembly of protein fusions to stem cells for cardiac therapy. Designing and optimising multi-module protein fusions to enhance the therapeutic performance of stem cells and their homing to infarcted tissue. These proteins contain one module that anchors to the cell membrane of stem cells by ionic interaction, whilst the other module is an acceptor for specific fusion to other proteins (e.g. fluorescent tags, transcription factors, etc.). https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4bd3dacbc08f59eeb57e0b/1582548749043/TomR.png Students - Dr Thomas Richardson My doctoral and postdoctoral work has centered around building a 3D model of the renal glomerular filtration barrier, using conditionally immortalised podocytes and endothelial cells first established at the University of Bristol. I broadly followed two approaches to achieve this aim, one using 3D bioprinting and the other using fibrin gels and protein engineering. In the latter, I co-developed a method generating 3D fibrin scaffolds from purified and modified thrombin. By chemically modifying thrombin so it had a surfactant corona, the enzyme could be embedded in the membrane of several cell types. In the presence of fibrinogen in solution, this membrane bound thrombin would form a hydrogel from the very surface of the cells, serving as a kind of extracellular matrix (ECM) mimic. Conditionally immortalised podocytes and endothelial cells in these 3D fibrin gels produced ECM specific to the glomerular basement membrane, which was verified using lightsheet microscopy. The second major aim of my PhD was to develop new 3D bioprinting methods to build a 3D renal model. I have also developed methodologies relating to fluorescence imaging (namely confocal microscopy), image processing, electron microscopy and immunohistochemistry. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4bd3e66ddc335ff290b021/1582296504233/WillM.jpg.png Students - William Macalester Periodontitis is an oral disease characterised by the persistent inflammation of periodontal tissues which, if left untreated, ultimately leads to alveolar bone dissolution and tooth loss. My project aims to create an in vitro model of the human periodontium in order to elucidate potential growth mechanisms which may inform the development of regenerative therapies. This work combines bioprinting and tissue engineering approaches to generate a 3D tissue model amenable to high throughput testing. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4c352eab57597f6de42deb/1582052688316/2020WIS.png Students https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4c41818b747830b7f58d5c/1582487412233/SaraM.png Students - Dr Sara Menegatti Trained as a Stem Cell Biologist, I joined CytoSeek and its mission to improve cell therapies by using a unique cell membrane augmentation technology. My aim is to develop in vitro and in vivo assays to assess the efficacy of our therapeutic in the fields of oncology and regenerative medicine. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4c41808861a57e42526419/1582296503627/AndreaDG.png Students - Andrea Diaz Gaxiola I am working on the manufacturing of light-controlled soft robots. These are not the classically known robots made of steel and wires. On the contrary, they are hydrogels made soft and squishy, yet tough and elastic. The greatest feature is their application as smart scaffolds for tissue engineering. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4c41ff2b169d25f3733085/1582480894463/person_teal.png Students - Agata Jakimowicz My research project is focused on the development of a smart microporous biological 3D printable ink (bioink) that can be used in both in vitro and in vivo tissue engineering. The structural component of the bioink is a viscoelastic hydrogel that has a highly tuneable temperature-dependent phase transition. In practice, this allows it to be extruded from nozzles (e.g. a needle) as a liquid at room temperature, which subsequently transforms into a self-supporting hydrogel at body temperature. Housed within this biomaterial-based hydrogel, is a cell-free expression system, which can be developed to produce growth factors that are essential for bone healing. Significantly, the delivery of these factors will be controlled using transcription regulation, such that the growth factor expression levels can be systematically modulated. The resulting smart bioink will be 3D printed in the presence of mesenchymal stem cells to control differentiation. This method of stem cell differentiation stimulation into osteoblasts has potential as a powerful technique in tissue engineering research and as a clinical tool for bone fracture repair. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4c4ca320c52d785aaf880d/1582296503631/ Students - Dr Graham Day My project involves the biophysical and structural characterisation of phosphotriesterases, which hydrolyse neurotoxic organophosphorus compounds found in chemical warfare agents and pesticides. Moreover, these enzymes are re-engineered to facilitate complexation to polymer surfactants to generate hierarchically self-assembled materials such as films or composite textiles. These robust materials exhibit the properties of the constituent proteins and offer new solutions to protection from and decontamination of organophosphorus compounds. I am also exploring new enzyme–biopolymer conjugation strategies to increase the catalytic activity or substrate range of these enzymes. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4c4e061a75040c0b0c3873/1589196472475/Person_rose.png Students - Ioannis Zampetakis The research project focuses on the development of novel composite materials using Cactus (Opuntia ficus-indica) fibres for impact energy dissipation and bone tissue engineering applications. This type of natural fibres has shown interesting deformation mechanics and energy dissipating potential under cyclic fatigue loading. We aim to characterize the natural fibres on a multiscale level to gain an understanding of their mechanical properties and use them as design guidelines for the development of novel polymeric composites and assessing their potential in energy dissipation and bone regeneration applications. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4c531b9acc280f2920cf7b/1582487457364/Person+gray.png Students - Thomas Taylor Cytoseek Partnered Project https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4c53271a75040c0b0d8114/1582487436350/Person_white.png Students - Ximena Vasto Anzaldo Cytoseek Partnered Project. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4d80a33f73540486ffaaac/1582548657651/ Students - Dr Sara Correia Carreira I'm developing the next generation of laboratory models of human skin with the aim of reducing animal testing. A major limitation of current skin models is that they don’t mimic the stretching and bending that skin experiences in real life. It is important to incorporate these motions because they can influence how substances penetrate through skin, or how wearable devices perform when skin is moving. In my research, I'm interfacing tissue engineered skin with soft robotic actuators, which function like artificial muscles that can stretch and bend the skin. Delivering mechanical stimulation to lab-grown skin can also improve the quality of skin grafts used to treat burns and wounds. Currently, these grafts are grown in the lab without ever experiencing the mechanical stresses they will encounter once they are applied to the patient. This means they don’t build up the toughness and structure of natural skin, which may be a reason for skin graft failures, such as tears. Therefore, I am also working towards engineering better skin grafts. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4d83d35864f4372b4903e7/1582983018422/ Students - Dr Ben Carter I worked in the Perriman Group from September 2014 – March 2018. My PhD, entitled 'Constructs for the detoxification of organophosphorous compounds', involved developing the first artificial membrane-binding enzymes. Using OpdA, an OP-degrading enzyme that evolved in soil bacteria, I was able to generate enzymatically-active constructs that spontaneously bound to cell membranes. I now work for CytoSeek, a spin-out from the Perriman group that is commercialising artificial membrane-binding protein technology to address unmet needs in cell-therapy treatments for cancers. For more information, visit cytoseek.uk. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4ec0020a541b68f725b764/1582548667843/JennaS.jpg Students - Dr Jenna Shapiro https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4fdb65db6bdf4d944f8835/1582296503520/DavidC.png Students - Dr David Coe https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4fdee0e28b674d653fd273/1582982633434/ Students - Professor Adam Perriman https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4fee041387b9765714f244/1582710414973/TomG.png Students - Dr Thomas Green As a PhD student in the Perriman Lab I was primarily interested in augmenting the functionality of stem cells without modifying their genomes; particularly focusing on homing to damaged heart tissue following myocardial infarction. I accomplished this by constructing an artificial membrane binding molecule which incorporated a repurposed bacterial adhesion molecule. This novel construct bound to immobilized fibronectin with greater specificity and affinity than a comparable antibody, and demonstrated increased mesenchymal stem cell homing to cardiac tissue in a mouse model. This methodology paved the way for further experiments in the group using similar systems. After completing my PhD research I joined Professor Perriman’s spin-out company Cytoseek as a founding member and now work towards commercialising the technology developed by myself and others in the academic group https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e555ff921592f1f698f65f7/1582807394026/MedelineBurke.png Students - Dr Madeline Burke My project which encompassed a wide range of interdisciplinary topics, including scaffold design for tissue engineering, the culture of disease models for kidney fibrosis, and 3D bioprinting of cells. Successfully graduated in 2017 and am now working as a Science Policy advisor in Westminster https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e556001c253f84cdc25b70c/1582653449329/KirsLeVay.png Students https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e556121035f7f739e97dfd4/1582654742201/JamesArmstrong.png Students - Dr James Armstrong https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e5564e7f4d63a75132e73d0/1582655216430/MonikaJakimowicz.png Students - Dr Monika Jakimowicz https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e5566cc0c0e830ca4ac804f/1582655266993/RosaliaCua.png Students - Dr Rosalia Cuahtecontzi https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e5568f27b18e31d4d100801/1582655783901/ Students - Dr Robert Deller https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/601d44e7e65d787cd60a869a/1612531497476/ValeriaS.jpg Students - Valeria Sandoval Torres Cytoseek Partnered Project - Adoptive Cell Therapies for Cancer https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e4e9de6f1b194361e7d39ac/1582210564467/labwork2.jpg Students https://www.perrimangroup.co.uk/pdra daily 0.75 2020-02-27 https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4bd3a971f6217a28f2949f/1612531278943/BethH.png Post-Doctoral Research Associates - Beth Hickton My project is a dstl industry-sponsored collaboration between the departments of Aerospace and Biomedical Engineering. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4bd3b0065dc80ce71c07a9/1583186349606/CorriganH.png Post-Doctoral Research Associates - Corrigan Hicks My project involves the development of artificial membrane binding proteins (AMBPs) for the modification of synthetic vesicles to enhance in vivo cardiovascular repair. My project involves the rational design and comprehensive biophysical characterisation of both the vesicles, AMBPs and modified vesicles. This work is a step towards building a modular homing system to target synthetic vesicles towards various specific sites in vivo. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4bd3bc47efbd6a69b7ed8d/1582984057384/GeorgeK.png Post-Doctoral Research Associates - George Klemperer My research work is in the area of 3D-printable engineered living materials (ELMs). ELMs are a type of biohybrid material that combine whole living cells with non-biological components/scaffolds. The idea is to combine the best of both worlds. The autonomous capabilities of cells with the tractability of synthetic materials (may they be polymers/gels/ceramics/metals etc.) to create materials with novel biologically-derived feedback properties and embedded intelligence. In my project, I aim develop a 3D printable E. coli-laden bioink ELM. This will become a new platform for patterned bacterial microreactor synthesis (constructs augmented with the abilities of transformed E. coli). Current bacterial microreactor applications being investigated include organophosphate bioremediation and hybrid gel synthesis. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4bd3c701c72f4e3e82aad6/1582296504724/MarkS.png Post-Doctoral Research Associates - Mark Shannon I’m working on the fabrication of engineered living materials towards the development of bioreactors. This project involves the 3D bioprinting of bacteria laden hydrogels that can be precisely deposited into predesigned architectures. These structures both shelter the cells and maintain their metabolic function. The genetic tractability of bacteria permits the expression of a wide range of recombinant enzymes to tailor these materials for bespoke application in bioremediation. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4bd3cd6d8433076e1b4ebc/1582296504120/PeterJ.png Post-Doctoral Research Associates - Peter Johnson 3D response of cancer to chemotherapies can be modelled using cancer spheroids, which grow from single cancer cells in a 3D matrix. High throughput bioprinting, coupled with high content microscopy and image analysis, allows for the screening of different therapeutic combinations against thousands of cancer spheroids in a controlled manner. In the future, this approach could be used to reduce the need for animals in drug testing, or to personalise cancer therapies for patients by analysing treatment combinations to find an optimal therapy. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4bd3ce79fe5b341b7d3b96/1582986311415/RaquelCS.jpg.png Post-Doctoral Research Associates - Dr Raquel Cruz Samperio Macromolecular assembly of protein fusions to stem cells for cardiac therapy. Designing and optimising multi-module protein fusions to enhance the therapeutic performance of stem cells and their homing to infarcted tissue. These proteins contain one module that anchors to the cell membrane of stem cells by ionic interaction, whilst the other module is an acceptor for specific fusion to other proteins (e.g. fluorescent tags, transcription factors, etc.). https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4bd3dacbc08f59eeb57e0b/1582548749043/TomR.png Post-Doctoral Research Associates - Dr Thomas Richardson My doctoral and postdoctoral work has centered around building a 3D model of the renal glomerular filtration barrier, using conditionally immortalised podocytes and endothelial cells first established at the University of Bristol. I broadly followed two approaches to achieve this aim, one using 3D bioprinting and the other using fibrin gels and protein engineering. In the latter, I co-developed a method generating 3D fibrin scaffolds from purified and modified thrombin. By chemically modifying thrombin so it had a surfactant corona, the enzyme could be embedded in the membrane of several cell types. In the presence of fibrinogen in solution, this membrane bound thrombin would form a hydrogel from the very surface of the cells, serving as a kind of extracellular matrix (ECM) mimic. Conditionally immortalised podocytes and endothelial cells in these 3D fibrin gels produced ECM specific to the glomerular basement membrane, which was verified using lightsheet microscopy. The second major aim of my PhD was to develop new 3D bioprinting methods to build a 3D renal model. I have also developed methodologies relating to fluorescence imaging (namely confocal microscopy), image processing, electron microscopy and immunohistochemistry. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4bd3e66ddc335ff290b021/1582296504233/WillM.jpg.png Post-Doctoral Research Associates - William Macalester Periodontitis is an oral disease characterised by the persistent inflammation of periodontal tissues which, if left untreated, ultimately leads to alveolar bone dissolution and tooth loss. My project aims to create an in vitro model of the human periodontium in order to elucidate potential growth mechanisms which may inform the development of regenerative therapies. This work combines bioprinting and tissue engineering approaches to generate a 3D tissue model amenable to high throughput testing. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4c352eab57597f6de42deb/1582052688316/2020WIS.png Post-Doctoral Research Associates https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4c41818b747830b7f58d5c/1582487412233/SaraM.png Post-Doctoral Research Associates - Dr Sara Menegatti Trained as a Stem Cell Biologist, I joined CytoSeek and its mission to improve cell therapies by using a unique cell membrane augmentation technology. My aim is to develop in vitro and in vivo assays to assess the efficacy of our therapeutic in the fields of oncology and regenerative medicine. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4c41808861a57e42526419/1582296503627/AndreaDG.png Post-Doctoral Research Associates - Andrea Diaz Gaxiola I am working on the manufacturing of light-controlled soft robots. These are not the classically known robots made of steel and wires. On the contrary, they are hydrogels made soft and squishy, yet tough and elastic. The greatest feature is their application as smart scaffolds for tissue engineering. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4c41ff2b169d25f3733085/1582480894463/person_teal.png Post-Doctoral Research Associates - Agata Jakimowicz My research project is focused on the development of a smart microporous biological 3D printable ink (bioink) that can be used in both in vitro and in vivo tissue engineering. The structural component of the bioink is a viscoelastic hydrogel that has a highly tuneable temperature-dependent phase transition. In practice, this allows it to be extruded from nozzles (e.g. a needle) as a liquid at room temperature, which subsequently transforms into a self-supporting hydrogel at body temperature. Housed within this biomaterial-based hydrogel, is a cell-free expression system, which can be developed to produce growth factors that are essential for bone healing. Significantly, the delivery of these factors will be controlled using transcription regulation, such that the growth factor expression levels can be systematically modulated. The resulting smart bioink will be 3D printed in the presence of mesenchymal stem cells to control differentiation. This method of stem cell differentiation stimulation into osteoblasts has potential as a powerful technique in tissue engineering research and as a clinical tool for bone fracture repair. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4c4ca320c52d785aaf880d/1582296503631/ Post-Doctoral Research Associates - Dr Graham Day My project involves the biophysical and structural characterisation of phosphotriesterases, which hydrolyse neurotoxic organophosphorus compounds found in chemical warfare agents and pesticides. Moreover, these enzymes are re-engineered to facilitate complexation to polymer surfactants to generate hierarchically self-assembled materials such as films or composite textiles. These robust materials exhibit the properties of the constituent proteins and offer new solutions to protection from and decontamination of organophosphorus compounds. I am also exploring new enzyme–biopolymer conjugation strategies to increase the catalytic activity or substrate range of these enzymes. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4c4e061a75040c0b0c3873/1589196472475/Person_rose.png Post-Doctoral Research Associates - Ioannis Zampetakis The research project focuses on the development of novel composite materials using Cactus (Opuntia ficus-indica) fibres for impact energy dissipation and bone tissue engineering applications. This type of natural fibres has shown interesting deformation mechanics and energy dissipating potential under cyclic fatigue loading. We aim to characterize the natural fibres on a multiscale level to gain an understanding of their mechanical properties and use them as design guidelines for the development of novel polymeric composites and assessing their potential in energy dissipation and bone regeneration applications. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4c531b9acc280f2920cf7b/1582487457364/Person+gray.png Post-Doctoral Research Associates - Thomas Taylor Cytoseek Partnered Project https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4c53271a75040c0b0d8114/1582487436350/Person_white.png Post-Doctoral Research Associates - Ximena Vasto Anzaldo Cytoseek Partnered Project. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4d80a33f73540486ffaaac/1582548657651/ Post-Doctoral Research Associates - Dr Sara Correia Carreira I'm developing the next generation of laboratory models of human skin with the aim of reducing animal testing. A major limitation of current skin models is that they don’t mimic the stretching and bending that skin experiences in real life. It is important to incorporate these motions because they can influence how substances penetrate through skin, or how wearable devices perform when skin is moving. In my research, I'm interfacing tissue engineered skin with soft robotic actuators, which function like artificial muscles that can stretch and bend the skin. Delivering mechanical stimulation to lab-grown skin can also improve the quality of skin grafts used to treat burns and wounds. Currently, these grafts are grown in the lab without ever experiencing the mechanical stresses they will encounter once they are applied to the patient. This means they don’t build up the toughness and structure of natural skin, which may be a reason for skin graft failures, such as tears. Therefore, I am also working towards engineering better skin grafts. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4d83d35864f4372b4903e7/1582983018422/ Post-Doctoral Research Associates - Dr Ben Carter I worked in the Perriman Group from September 2014 – March 2018. My PhD, entitled 'Constructs for the detoxification of organophosphorous compounds', involved developing the first artificial membrane-binding enzymes. Using OpdA, an OP-degrading enzyme that evolved in soil bacteria, I was able to generate enzymatically-active constructs that spontaneously bound to cell membranes. I now work for CytoSeek, a spin-out from the Perriman group that is commercialising artificial membrane-binding protein technology to address unmet needs in cell-therapy treatments for cancers. For more information, visit cytoseek.uk. https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4ec0020a541b68f725b764/1582548667843/JennaS.jpg Post-Doctoral Research Associates - Dr Jenna Shapiro https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4fdb65db6bdf4d944f8835/1582296503520/DavidC.png Post-Doctoral Research Associates - Dr David Coe https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4fdee0e28b674d653fd273/1582982633434/ Post-Doctoral Research Associates - Professor Adam Perriman https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e4fee041387b9765714f244/1582710414973/TomG.png Post-Doctoral Research Associates - Dr Thomas Green As a PhD student in the Perriman Lab I was primarily interested in augmenting the functionality of stem cells without modifying their genomes; particularly focusing on homing to damaged heart tissue following myocardial infarction. I accomplished this by constructing an artificial membrane binding molecule which incorporated a repurposed bacterial adhesion molecule. This novel construct bound to immobilized fibronectin with greater specificity and affinity than a comparable antibody, and demonstrated increased mesenchymal stem cell homing to cardiac tissue in a mouse model. This methodology paved the way for further experiments in the group using similar systems. After completing my PhD research I joined Professor Perriman’s spin-out company Cytoseek as a founding member and now work towards commercialising the technology developed by myself and others in the academic group https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e555ff921592f1f698f65f7/1582807394026/MedelineBurke.png Post-Doctoral Research Associates - Dr Madeline Burke My project which encompassed a wide range of interdisciplinary topics, including scaffold design for tissue engineering, the culture of disease models for kidney fibrosis, and 3D bioprinting of cells. Successfully graduated in 2017 and am now working as a Science Policy advisor in Westminster https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e556001c253f84cdc25b70c/1582653449329/KirsLeVay.png Post-Doctoral Research Associates https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e556121035f7f739e97dfd4/1582654742201/JamesArmstrong.png Post-Doctoral Research Associates - Dr James Armstrong https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e5564e7f4d63a75132e73d0/1582655216430/MonikaJakimowicz.png Post-Doctoral Research Associates - Dr Monika Jakimowicz https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e5566cc0c0e830ca4ac804f/1582655266993/RosaliaCua.png Post-Doctoral Research Associates - Dr Rosalia Cuahtecontzi https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/5e5568f27b18e31d4d100801/1582655783901/ Post-Doctoral Research Associates - Dr Robert Deller https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/5e4bd376518fab78b4f6faee/601d44e7e65d787cd60a869a/1612531497476/ValeriaS.jpg Post-Doctoral Research Associates - Valeria Sandoval Torres Cytoseek Partnered Project - Adoptive Cell Therapies for Cancer https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e4fd8b95860ac124da6a154/1582291160246/labwork4.jpg Post-Doctoral Research Associates https://www.perrimangroup.co.uk/cytoseek daily 0.75 2020-02-23 https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e52cc75fe69f273e6e653da/1582484602632/Tumour+zone_edited.jpg Cytoseek https://www.perrimangroup.co.uk/news daily 0.75 2021-02-08 https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/60218ea06224311fbf648e26/1612811950179/shutterstock_540587344_Microscope.jpg News - Postdoctoral Position Available We are looking for world-class researchers with a high degree of innovative spirit and enthusiasm to operate at the very cutting edge of robotics and healthcare research. A Postdoctoral Researcher (PDRA) position in tissue engineering for artificial muscle integration is available to support the activities of the University of Bristol and Bristol Robotics Laboratory on the £7 million ($9.6 million / €7.9 million) EPSRC-funded project ”emPOWER: in-body artificial muscles for physical augmentation, function restoration, patient empowerment and future healthcare”. This project will develop soft robotic, smart materials and bio-interfacing technologies for implantable artificial muscles. It will target critical global healthcare needs including stroke rehabilitation, reversing age-related frailty and sarcopenia and restoration of body functions which have been affected by muscle dysfunction. The project will run for five years in the first instance from March 2021 and the PDRA position is available for the full five-year length of the project. Learn more:Job Details & emPOWER https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e55a52f59a6300d9dbe89a8/1582671237933/labwork5_green.png News https://www.perrimangroup.co.uk/bloopers daily 0.75 2020-02-27 https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e5817d37b69d0173be8af0a/1582831661752/IMG_2955_edited.jpg Bloopers https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e581867bb9f3678e1154c1d/1582831764783/IMG_2645_edited.jpg Bloopers https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e5815afbb9f3678e1148d27/1582831027689/IMG_2948_edited.jpg Bloopers https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e5817765aea611485b0391b/1582831547444/IMG_2905_edited.jpg Bloopers https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e581693690ed84297c4e45b/1582831309153/IMG_2809_edited.jpg Bloopers https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e5816d26cd88a1e9f31d189/1582831368169/IMG_2817_edited.jpg Bloopers https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e5815a3597d93194a5b03a3/1582831016920/IMG_3119_edited.jpg Bloopers https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e5816174446937bba148fb8/1582831195508/IMG_2936.JPG Bloopers https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e58172cc54055341b6f05a9/1582831457758/IMG_2896_edited.jpg Bloopers https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e5815bc5aea611485afc78c/1582831040467/IMG_2995_edited.jpg Bloopers https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e5815de7b69d0173be82f49/1582831101751/ Bloopers https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e58193ca337185b1367ed82/1582831948639/IMG_2973.jpg Bloopers https://www.perrimangroup.co.uk/404 daily 0.75 2020-02-29 https://static1.squarespace.com/static/5d3b0ea2b28c1b0001f1084a/t/5e5a6dbe483fcf203324575f/1582984645973/404.png Content not found or URL mistake…